Multi -Fox Microscope Fast Live 3D leads to biological imaging limits

Researchers have developed a high -speed imaging microscope that can capture the detailed cell dynamics of the entire small organism at the same time. The ability to create a picture of 3D changes in a large field of theory can lead to new insights in development biology and neuro science.

Edward Hirata Miasaki said, “Traditional microscopes is forced to re -focus or scan at different depths, which makes it difficult to capture a fast, 3D biological process without any distortion or missing information,” said Edward. (Ustriya California Sant Cruz) Bio -Hub.

“Our new system expands the multi -focus microscopy (MFM) technique, which Ibrahimson has developed using 25 camera array to advance the limits of speed and volume. It opens the door to study small residential systems without interrupting them in performance.”

I OpticaResearchers, researchers, describe their new microscope, which combines disparity optics with 25 small cameras with sync and simultaneously deepening. They demonstrate direct imaging of 3D volumes of 25 aircraft, which is measured up to 180 x 180 x 50 micron, with more than 100 volumes per second.

Herita Miasaki said, “The new microscope, which we call M25, is especially useful for imaging swimming segments worms, a model biology that is used to study development, neuro science and locomotions.”

“Traditionally, scientists could only see a part of the organism at any time. With our new microscope, it is possible to see the whole insect naturally in 3D, allowing researchers to study how its nervous system moves and how to treat genetic variation, disease or drug treatment.”

Multi -aircraft Light Control

An important part of the new microscope is different optical elements used to distribute different focal aircraft into a row of 25 cameras. Discrimination Optics use micro -structure uses the use of manipulation in light, which allows more complex light control through traditional optical components such as more thin, light component.

While highlighting the original MFM technique, researchers designed a multi -focus greeting to distribute incoming light so that each camera can capture the same scene, but its focus is on a different depth. He also did custom greeting to use in front of each camera lens to correct the colorfulness introduced by multi -focus greeting.

By replacing the traditional colorful correction, which was difficult to scal on a scale of more than 3 × 3 rows, these flames enabled high -speed biomeding in more aircraft.

Griving is made of nanometer scale samples that require special fabric tools. After using imitation to determine maximum designs, researchers used the University of California Santa Barbara Nano Foabcation to remove glass samples in glass. With the formation of the fabricated process, these different elements can be re -reproduced in a high quantity.

Ibrahimson said, “One of the key innovations of M25 is the use of easy colorful correctional architecture. By converting very large -based components with custom designed bleeding gravites, the system achieves efficient dispersion in all focal aircraft while compact and expansion.”

“This smooth optical design not only enables high -speed imaging but also supports compatibility with label ways.

Researchers also developed a new software to handle data from 25 different cameras simultaneously to handle the challenge of rapidly integrating and obtaining the challenge of storing it in a computer.

“When jointly, 25 images – all were obtained simultaneously, with no mechanical scanning or moving parts – create a full 3D snapshot,” said Herata Miasaki. “Since it is fast, only the camera acquisition is limited by the speed and the brightness of the sample, so we can record the whole volumes over time, and enable the study of real biological dynamics.”

Accessible and versatile imaging

The M25 microscope can be used for both fluorosis and label -free methods, such as luminous field and polarization microscopy, which are especially useful for imaging sensitive biological systems without introducing colors or labels. With the least invasive techniques, this compatibility makes the M25 appropriate to the applications such as embryology, where the protection of ancestral physicality is essential.

To verify the device, researchers made a prototype and confirmed that it could get 25 separate, uniformly distance focal planes simultaneously through imaging, through imaging of calibration goals, without distortion or overlap.

He also used microscope directly to create a picture of biological samples, including ordinary model organisms such as C -melanogaster and P Marines, which demonstrated the realtime 3D imaging of moving organisms without scanning or motion compensation.

The system is mounted on the standard commercial microscope side port. In addition to discrepancy optics, it does not require any special hardware, which makes it more straightforward to produce more duplication than customs or complex lighting modification systems.

Detailed fabric measures for the preparation of color correction and multi -foxes used in the M22 3D imaging system are available. These ingredients can be fabricated on any educational nano -fabrication facility, including the UCSB Nano FABification Facility.

Acquisition engines and nephew plugin are available on the gut hub.

After that, the researchers aim to further enhance the scale and applications of the system. For example, they intend to use the system’s full imaging data to train machine learning models that can identify cell states, track dynamic behavior, and detect direct disease changes through images.